Translational_Unit

Part:BBa_K190020:Design

Designed by: Nienke Kuipers   Group: iGEM09_Groningen   (2009-08-19)

MymT


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BioBrick prefix and suffix was added by PCR, also the RBS (B0034) was added by PCR (not in the sequence yet).


Source

MymT was cloned from pGB68 (kindly provided by Ben Gold, Cornell University), the gene's origin is the genome of M. tuberculosis

References

Identification of a copper-binding metallothionein in pathogenic mycobacteria. Ben Gold et al. Nature Chemical Biology, Volume 4 nr 10, Oct 2008